APPLICATION NOTE
Sensitive quantitation of host cell protein contamination of biologics manufactured in CHO cells
Host cell proteins (HCPs) are produced by the cells in which biologics are manufactured. Although they may be vital to the health or growth of these cells, they are not related to the biologic itself and must be removed from the final product.
Detection and removal of HCP is a time-consuming process, and levels of HCP must be monitored throughout the purification process to ensure safety of the final product.
In this application note we showcase how the automation of wash steps using the AquaMax® 4000 Microplate Washer reduces hands-on time, accelerating the workflow with no compromise in data quality. Absorbance was detected using a SpectraMax® iD3 Multi-Mode Microplate Reader, and data were analyzed using SoftMax® Pro Software, which enables automated standard curve graphing and sample data analysis.
Heading
Download Application Note
Product family
Microplate Reader
Product primary application
AquaMax Microplate Washer; SpectraMax iD3 Multi-Mode Microplate Reader; SoftMax Pro GxP Software;SoftMax Pro Software
CMP
7014u0000017fHZAAY
Title
Sensitive quantitation of host cell protein contamination of biologics manufactured in CHO cells
Resource URL
Resource Image URL
