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Mesure de plusieurs longueurs d’onde sans filtre

Les lecteurs de microplaques Gemini™ XPS et EM à double monochromateur offrent un environnement flexible pour déterminer les paramètres d’excitation et d’émission optimaux pour vos tests d’intensité de fluorescence. L'analyse de puits à points multiples optimise la sensibilité des tests cellulaires. La comparaison des unités de fluorescence relative (RFU) entre échantillons est possible grâce à un étalonnage unique par rapport à une norme interne. Les réactions sensibles à la température sont surveillées avec une régulation de température homogène allant de la température ambiante à 45 °C.

  • Il n’est plus nécessaire de changer les filtres

    Il n’est plus nécessaire de changer les filtres

    Il n’est plus nécessaire d’identifier, d'acheter ni de changer les filtres. Les doubles monochromateurs permettent de choisir la longueur d’onde d’excitation et d’émission entre 250 et 850 nm.

  • Mesure plus précise

    Mesure plus précise

    Balayage d’un puits pour rapporter une mesure de fluorescence d’un seul point au centre du puits de la microplaque à de multiples points à travers ce puits traité pour la culture tissulaire.

  • Plus de perte de signaux élevés

    Plus de perte de signaux élevés

    Évite la perte de signaux élevés due à la saturation du détecteur et trouve les paramètres de lecture corrects grâce à notre système d’optimisation AutoPMT breveté.

Gemini

Gemini

Caractéristiques

  • Icône Lecture par le dessus

    Capacité de lecture par le haut

    Le lecteur de microplaques de lecture par le haut Gemini XPS mesure la fluorescence sur un grand nombre de formats d’échantillon à partir de microplaques de 6 à 384 puits en modes point final, cinétique, balayage spectral et analyse de puits.

  • Icône Haut et bas

    Capacité de lecture par le haut et par le bas

    Le lecteur de microplaques de lecture par le haut et par le bas Gemini XPS mesure la fluorescence sur un grand nombre de formats d’échantillon à partir de microplaques de 6 à 384 puits en modes point final, cinétique, balayage spectral et analyse de puits.

Applications des lecteurs de microplaques Gemini XPS et EM

Caractéristiques et options des lecteurs de microplaques Gemini XPS et EM

 

*En utilisant si possible la vitesse de lecture et les paramètres les plus bas .

Ressources sur les lecteurs de microplaques Gemini XPS et EM

  • Citation
    Dated: May 01, 2011
    Publication Name: Bentham Science Publishers

    Amino Acid-Substituted Gemini Surfactant-Based Nanoparticles as Safe and Versatile Gene Delivery Agents

    Gene based therapy represents an important advance in the treatment of diseases that heretofore have had either no treatment or cure. To capitalize on the true potential of gene therapy, there is a need to develop better delivery systems that can protect these therapeutic biomolecules and deliver them safely to the target sites. Recently, we have… View more

    Gene based therapy represents an important advance in the treatment of diseases that heretofore have had either no treatment or cure. To capitalize on the true potential of gene therapy, there is a need to develop better delivery systems that can protect these therapeutic biomolecules and deliver them safely to the target sites. Recently, we have designed and developed a series of novel amino acid-substituted gemini surfactants with the general chemical formula C12H25(CH3)2N+- (CH2)3-N(AA)-(CH2)3-N+(CH3)2-C12H25 (AA= glycine, lysine, glycyl-lysine and, lysyl-lysine). These compounds were synthesized and tested in rabbit epithelial cells using a model plasmid and a helper lipid. Plasmid/gemini/lipid (P/G/L) nanoparticles formulated using these novel compounds achieved higher gene expression than the nanoparticles containing the parent unsubstituted compound. In this study, we evaluated the cytotoxicity of P/G/L nanoparticles and explored the relationship between transfection efficiency/toxicity and their physicochemical characteristics (such as size, binding properties, etc.). An overall low toxicity is observed for all complexes with no significant difference among substituted and unsubstituted compounds. An interesting result revealed by the dye exclusion assay suggests a more balanced protection of the DNA by the glycine and glycyl-lysine substituted compounds. Thus, the higher transfection efficiency is attributed to the greater biocompatibility and flexibility of the amino acid/peptide-substituted gemini surfactants and demonstrates the feasibility of using amino acid-substituted gemini surfactants as gene carriers for the treatment of diseases affecting epithelial tissue.

    Contributors: Singh, Jagbir; Yang, Peng; Michel, Deborah; E. Verrall, Ronald; Foldvari, Marianna; Badea, Ildiko  
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  • Citation
    Dated: Feb 24, 2010
    Publication Name: ASSAY and Drug Development Technologies

    A Human CXCL13-Induced Actin Polymerization Assay Measured by Fluorescence Plate Reader

    The chemokine receptor CXCR5 is predominantly expressed on mature B cells and follicular T-helper cells. CXCR5 and its ligand CXCL13 participate in ectopic germinal center formation at the inflammatory sites of multiple immune diseases such as rheumatoid arthritis, multiple sclerosis, and Sjogren’s syndrome. Therefore, disrupting CXCL13-induced… View more

    The chemokine receptor CXCR5 is predominantly expressed on mature B cells and follicular T-helper cells. CXCR5 and its ligand CXCL13 participate in ectopic germinal center formation at the inflammatory sites of multiple immune diseases such as rheumatoid arthritis, multiple sclerosis, and Sjogren’s syndrome. Therefore, disrupting CXCL13-induced chemotaxis may be a fruitful approach for developing therapeutics in treating these diseases. Cells undergo cytoskeletal rearrangement prior to chemotaxis, and therefore actin polymerization can be used as a surrogate readout more proximal to chemokine receptor activation than chemotaxis. Conventionally, actin polymerization is measured by fluorescence microscopy or flow cytometry, which are either of low throughput or in need of special instruments. We developed a 96-well actin polymerization assay that can process 1,000 to 1,500 samples a day. This assay uses a standard laboratory fluorescence microplate reader as the detection instrument and was optimized for various experimental conditions such as cell density, actin filament staining reagent, staining buffer, and cell culture conditions. We demonstrate that this actin polymerization assay in 96-well format exhibits the expected pharmacology for human CXCR5 and is suitable as a primary functional assay to screen neutralizing scFv in crude bacterial peri-preps and a secondary assay for small compound collections.

    Contributors: Jennifer Alley, Laird Bloom, Marion Kasaian, Huilan Gao, Gabriel Berstein, James D. Clark, and Wenyan Miao  
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  • Citation
    Dated: May 09, 2005
    Publication Name: Journal of Materials Chemistry

    Phosphorescent oxygen-sensitive materials for biological applications

    A number of macromolecular probes employing different carriers and a number of microparticular probes employing different oxygen sensitive dyes were fabricated, giving a panel of oxygen sensitive probes. The photophysical and oxygen sensing properties of these probes were examined comparatively. The probes were used successfully to monitor… View more

    A number of macromolecular probes employing different carriers and a number of microparticular probes employing different oxygen sensitive dyes were fabricated, giving a panel of oxygen sensitive probes. The photophysical and oxygen sensing properties of these probes were examined comparatively. The probes were used successfully to monitor cellular oxygen uptake and their ability to overcome a number of problems associated with oxygen sensing in biological samples was assessed. Macromolecular probes proved sufficient in a number of cases, particularly where spectral solutions can resolve the interferences. However where physical interactions cause interference the added protection of the polymer in the particle based probes was required.

    Contributors: Ciara O'Donovan, James Hynes, Dmitri Yashunski and Dmitri B. Papkovsky  
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Lecteur de microplaques Gemini™ XPS

Produit

Numéro de produit

Lecteur de plaques Gemini XPS XPS
Plaque de validation de fluorescence SpectraTest FL1 0200-5060
Kit de démarrage à microplaques micro-volume SpectraDrop™ 0200-6262
Kit HTS microvolume SpectraDrop™ 0200-6267
Empileur de microplaques StakMax StakMax
Étagère de lecteur de microplaques 9000-0756

Lecteur de microplaques Gemini™ EM

Produit

Numéro de référence de la pièce

Lecteur de plaques Gemini EM EM
Plaque de validation de fluorescence SpectraTest FL1 0200-5060
Kit de démarrage à microplaques micro-volume SpectraDrop™ 0200-6262
Kit HTS à microplaques micro-volume SpectraDrop™ 0200-6267
Étagère de lecteur de microplaques 9000-0756

Produits et services connexes aux lecteurs de microplaques Gemini XPS et EM