FLIPR Tetra High-Throughput Cellular Screening System

Number of Citations*: 14,000

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FLIPR: a new instrument for accurate, high throughput optical screening

KS Schroeder, BD Neagle - Journal of Biomolecular …, 1996 -

Modern optical screening assays demand high data throughput along with uncompromised data fidelity. FLIPR (Fluorescent Imaging Plate Reader) was developed to perform quantitative optical screening for cell-based kinetic assays. FLIPR incorporates an …

Cited by 213 Related articles All 2 versions [PDF] 

c-FLIPR, a new regulator of death receptor-induced apoptosis

A Golks, D Brenner, C Fritsch, PH Krammer… - Journal of Biological …, 2005 - ASBMB

Abstract c-FLIPs (c-FLICE inhibitory proteins) play an essential role in regulation of death receptor-induced apoptosis. Multiple splice variants of c-FLIP have been described on the mRNA level; so far only two of them, c-FLIP L and c-FLIP S, had been found to be expressed …

Cited by 309 Related articles All 10 versions [PDF]

Measurement of [Ca2+] Using the Fluorometric Imaging Plate Reader (FLIPR)

E Sullivan, EM Tucker, IL Dale - Calcium Signaling Protocols, 1999 - Springer

Ionized calcium plays a major role in the regulation of cellular processes in both eukaryotic and prokaryotic cells (1). A wide variety of cell surface receptors and ion channels utilize a calcium signal to initiate events such as cell motility, contraction, and secretion. To a large …

Cited by 167 Related articles All 6 versions

Characterization of the mouse cold‐menthol receptor TRPM8 and vanilloid receptor type‐1 VR1 using a fluorometric imaging plate reader (FLIPR) assay

HJ Behrendt, T Germann, C Gillen… - British journal of …, 2004 - Wiley Online Library

TRPM8 (CMR1) is a Ca2+‐permeable channel, which can be activated by low temperatures, menthol, eucalyptol and icilin. It belongs to the transient receptor potential (TRP) family, and therefore is related to vanilloid receptor type‐1 (VR1, TRPV1). We tested whether …

Cited by 425 Related articles All 8 versions [TEXT] wiley.comFull View

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Characterization using FLIPR of rat vanilloid receptor (rVR1) pharmacology

JC Jerman, SJ Brough, R Prinjha… - British journal of …, 2000 - Wiley Online Library

The vanilloid receptor (VR1) is a ligand‐gated ion channel, which plays an important role in nociceptive processing. Therefore, a pharmacological characterization of the recently cloned rat VR1 (rVR1) was undertaken. HEK293 cells stable expressing rVR1 (rVR1‐HEK293) …

Cited by 130 Related articles All 6 versions  [TEXT] Full View

Characterisation using FLIPR of human vanilloid VR1 receptor pharmacology

D Smart, JC Jerman, MJ Gunthorpe, SJ Brough… - European journal of …, 2001 - Elsevier

A full pharmacological characterisation of the recently cloned human vanilloid VR1 receptor was undertaken. In whole-cell patch clamp studies, capsaicin (10 μM) elicited a slowly activating/deactivating inward current in human embryonic kidney (HEK293) cells stably …

Cited by 107 Related articles All 5 versions

Characterization of recombinant human orexin receptor pharmacology in a Chinese hamster ovary cell‐line using FLIPR

D Smart, JC Jerman, SJ Brough… - British journal of …, 1999 - Wiley Online Library

The cellular mechanisms underlying the physiological effects of the orexins are poorly understood. Therefore, the pharmacology of the recombinant human orexin receptors was studied using FLIPR. Intracellular calcium ([Ca2+] i) was monitored in Chinese hamster …

Cited by 181 Related articles All 7 versions [TEXT] Full View

Validation of FLIPR membrane potential dye for high throughput screening of potassium channel modulators

KL Whiteaker, SM Gopalakrishnan… - Journal of …, 2001 -

A fluorescence-based assay using the FLIPR Membrane Potential Assay Kit (FMP) was evaluated for functional characterization and high throughput screening (HTS) of potassium channel (ATP-sensitive Ki channel; KATP) modulators. The FMP dye permits a more …

Cited by 94 Related articles All 4 versions [PDF]

Evaluation of SDF‐1/CXCR4‐induced Ca2+ signaling by fluorometric imaging plate reader (FLIPR) and flow cytometry

K Princen, S Hatse, K Vermeire… - Cytometry Part A …, 2003 - Wiley Online Library

Background The chemokine receptors CXCR4 and CCR5 are the main coreceptors for human immunodeficiency virus (HIV) 1 to enter its target cells. The antiviral activity of their natural ligands (stromal cell‐derived factor 1 [SDF‐1], regulated on activation normal T‐cell …

Cited by 100 Related articles All 7 versions [PDF] Full View

Rapid assessment of ligand actions with nicotinic acetylcholine receptors using calcium dynamics and FLIPR

TA Kuntzweiler, SP Arneric… - Drug development …, 1998 - Wiley Online Library

Due to the complex mechanism of cation transport by neuronal nicotinic acetylcholine receptors (nAChR), potential ligands of these receptors must be characterized with respect to functional activity and corresponding subtype selectivity. Conventional radioactive binding …

Cited by 48 Related articles All 2 versions

Number of Citations*: 14,000

Latest Citations: For a complete list, please click here.