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AgileOptix Spinning Disk Technology

 

AgileOptix spinning disk technology

Our proprietary AgileOptix™ spinning disk technology is a microscopy innovation designed to run more complex, physiologically-relevant cellular models including 3D spheroids, tissues, live-cell assays and whole organisms. This technology combines the clarity of confocal imaging with the throughput and well-known robustness of the ImageXpress® Micro platform.

 

 

How the spinning disk technology works

One aspect of AgileOptix technology is a spinning disk light path, which includes a very bright solid state light engine. Excitation light passes through a rapidly spinning disk with symmetrically placed spirals of pinholes or slits. These pinholes or slits split the illumination light into multiple beams that scan the fluorescent sample. Emission light from the sample then passes back through the confocal disk and is then directed via a dichroic beamsplitter through the emission filter to a high-sensitivity scientific CMOS camera.

AgileOptix spinning disk confocal technology

 

The AgileOptix technology difference

Unlike other spinning disk confocal technologies used for high-content screening, AgileOptix technology offers the ability to adapt disk geometries to meet your unique assay requirements. It is one of the few technologies on the market that includes a unique dual disk configuration with both pinholes or slits to accommodate high-throughput applications.

 

AgileOptix technology enables the ImageXpress® Micro Confocal High-Content Imaging System to deliver the sensitivity and throughput needed for demanding applications by combining:

  • Powerful solid-state light engine
  • High-quantum efficiency 16-bit, >4 megapixel scientific CMOS sensor
  • >3 log dynamic range in both widefield and confocal modes
  • Large field of view (1,96 mm2 at 10X) maximizes collection of publication quality images and statistically relevant data
  • Specially designed optics to ensure the best images for your assay
  • Air objectives with up to 0,95 NA
  • Water objectives with up to 1,2 NA

 


 

Multiple confocal disk configurations for optimized assay performance

AgileOptix proprietary spinning disk technology includes simple, user-defined configurations to ensure maximum assay performance for both confocal and widefield imaging. Multiple confocal disk geometries are available, including pinhole and slit configurations.

 

 

Spinning disk geometry 60 µm pinhole
(single disk)
60 µm dual disk
with 50 µm slit
60 µm dual disk
with 42 µm pinhole
High-sensitivity detection
 
 
 
Fast acquisition
 
 
 
>3 log dynamic range†
 
 
 
Widefield mode for flat biology
 
 
 
Most confocal applications
 
 
 
Highest resolution imaging    
 
High-throughput applications  
 
 

*Powered by our highly responsive sCMOS sensor and advanced solid state light source.

 


 

Gain deeper insights with water immersion objectives

Objectifs de l’immersion dans l’eau

Water immersion objectives can offer signal increases of up to 4X** which can help scientists see deeper into 3D and thick tissue samples at lower exposure times.

  • Augmentez les profondeurs de pénétration dans les échantillons
  • Améliorez la résolution Z et les aberrations sphériques
  • Obtenez des images plus nettes
  • Accédez à encore plus de données qu’auparavant

En savoir plus  

Air
Objectifs immergés dans l’eau
Water
Objectifs de l’immersion dans l’eau

Images prises avec la même exposition.

**Data and images were acquired during development using customer samples. Les résultats peuvent varier.


*High-performance 3D customizations

AgileOptix technology offers the flexibility to add high-performance customizations. Water immersion objectives, combined with our custom deep tissue confocal disk and high-intensity lasers described below, allow you to penetrate even deeper into your 3D samples and capture sharper, crisper images.

Molecular Devices can successfully tailor the ImageXpress Micro Confocal system to include customized software and hardware including the features described herein, as well as integration of other lab components such as incubators, liquid handlers, and robotics for a fully automated workcell. Avec plus de 30 ans d'expérience dans l'industrie des sciences de la vie, vous pouvez compter sur nous pour fournir des produits de qualité et une assistance partout dans le monde.
 
La vente est soumise à nos Conditions d’achat de produits personnalisés disponibles sur www.moleculardevices.com/custom-products-purchase-terms

 

Lasers haute intensité

High output laser excitation can reduce exposure times by up to 75%.** The laser light source is available as either a 5-channel or 7-channel with outputs of 400 —1 000 mW/channel. The 7-channel laser light source Includes near IR and is ideal for customers with increased multiplexing requirements.

  • Obtenez des images plus nettes avec un rapport signal/bruit supérieur
  • Generate up to a 2X** boost in scan speed attributed to significantly reduced exposure times
  • Effectuez des expériences FRET avec des lasers pour CFP et YFP
Standard
Image d’intensité standard
Laser
Lasers haute intensité

Images prises avec la même exposition.

**Data and images were acquired during development using customer samples. Les résultats peuvent varier. Le tarif des caractéristiques mises en avant, le délai de livraison et les caractéristiques varient selon les exigences techniques convenues ensemble. Ces exigences peuvent entraîner des changements de la performance standard.

Module à disque confocal de pénétration des tissus profonds

An upgrade to the deep tissue penetrating, confocal disk module, combined with a laser light source, can improve resolution when imaging thick tissue samples.**

  • Améliorez la suppression de la lumière floue
  • Réduisez le trouble (lumière parasite sténopéique)
  • Pénétrer plus profondément dans les échantillons de tissu épais pour obtenir des images plus nettes
Disque rotatif standard
Deep tissue penetrating, confocal tissue module
Deep tissue penetrating, confocal tissue module
Confocal Tissue Module

Images prises avec la même exposition.

**Data and images were acquired during development using customer samples. Les résultats peuvent varier. Le tarif des caractéristiques mises en avant, le délai de livraison et les caractéristiques varient selon les exigences techniques convenues ensemble. Ces exigences peuvent entraîner des changements de la performance standard.

Resources for AgileOptix Spinning Disk Technology

Vidéos et webinaires

Objectives à immersion dans l’eau et imagerie à  haut contenu

Approfondissez vos connaissances sur les structures cellulaires 3D grâce à des objectifs à immersion dans l’eau