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Application Note

Detection and quantitation of protein with ScanLater Western Blot Detection System

  • Western blot protocol is unchanged except for incubation with secondary antibody
  • No substrates required
  • TRF detection using Europium-labeled secondary antibody reduces background while increasing dynamic range
  • Digital photon counting provides unaltered TRF counts
  • Europium resists photo bleaching and is stable for weeks, enabling reuse of blots