APPLICATION NOTE
Automated testing for compound toxicity effects using healthy intestinal organoids
Reduce manual processes and increase productivity and throughput for toxicity assessment studies with machine learning-enabled automation.
The most common side effects of anti-cancer drugs is their toxic effect to intestinal cells which often limits treatment dosage for patients. In vitro assays using three-dimensional (3D) organoids can evaluate toxicity effects to the intestine and provide essential information in the process of drug development. In addition, assay automation will greatly increase productivity and scale of these models, as well as the accuracy of assays that involve complex 3D biology.
Here, we describe how to automate the entire cell culture process of the compound testing assay for toxicity using 3D mouse intestinal organoids cultured in Matrigel domes. This workflow maintains a 24/7 schedule to efficiently scale up various cell models, cell lines, iPSCs, spheroids, or organoids.
