THE AXON GUIDE
Electrophysiology and Biophysics Laboratory Techniques
What you will find in this guide
This guide serves as an information and data resource for electrophysiologists. It covers a broad range of topics ranging from the biological basis of bioelectricity and a description of the basic experimental setup to a discussion of mechanisms of noise and data analysis.
The Axon Guide Fifth Edition is a tool benefiting both the novice and the expert electrophysiologist. Newcomers to electrophysiology will gain an appreciation of the intricacies of electrophysiological measurements and the requirements for setting up a complete recording and analysis system. For experienced electrophysiologists, we include in-depth discussions of selected topics, such as advanced methods in electrophysiology and noise.
For the fifth edition, we are very pleased to be able to offer new content in the Advanced Methods in Electrophysiology section, including advanced methods developed for ion channel recording from liposomes, planar bilayers, and bacterial spheroplasts; current recording from solid-state nanopores, and the use of nanopipettes in intracellular recording.
Table of contents
- 1. Bioelectricity
- 2 The Laboratory Setup
- 3. Instrumentation for Measuring Bioelectric Signals from Cells
- 4. Microelectrodes and Micropipettes
- 5. Advanced Methods in Electrophysiology
- 6. Signal Conditioning and Signal Conditioners
- 7. Transducers
- 8. Laboratory Computer Issues and Considerations
- 9. Acquisition Hardware
- 10. Data Analysis
- 11. Noise in Electrophysiological Measurements
- Appendix A: Guide to Interpreting Specifications
THE AXON GUIDE
The most comprehensive laboratory techniques workbook—with over 250 pages—to support electrophysical and biophysical research
Learn more about the Axon Instruments® portfolio:
Axon Instruments Patch-Clamp Amplifiers
Patch-clamp amplifiers from single channel, whole-cell, and two electrode recordings
- Control via pCLAMP software maximizes flexibility in setting up and performing different experimental protocols
- A wide range of amplifiers means we can offer the ideal choice for your application, providing the best possible data
- Our lowest-noise amplifier/digitizer instruments provide the highest signal resolution, and eliminate 50 or 60 Hz line-frequency noise contamination
The Axon™ Digidata® 1550B plus HumSilencer®
High-resolution, low-noise digitizer with 50/60 Hz line-frequency noise elimination
- High rate of sampling (500kHz) means a more faithful digitization process, allowing for precise digital reconstruction of the analogue signal due to minimal information loss
- Eight channels can be sampled and digitized simultaneously, increasing data acquisition speed
- The HumSilencer option with the Digidata range eliminates of 50 and 60Hz line-frequency noise, making the biological signal clearer without distorting the data
pCLAMP 11 Software Suite
A complete workflow solution for patch-clamp electrophysiology
- Enhanced protocol editor in pCLAMP 11 Software allows for longer and more sophisticated protocols to be programmed and reduces protocol setup time
- Multiple windows can be simultaneously displayed during a Membrane Test protocol, making it easier to patch multiple cells
- Gap-free mode allows the programming of command and digital outputs in a continuous recording, allowing you to enhance protocol design and trigger actions within the protocol automatically
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